|
KMID : 0603820140200020070
|
|
Journal of Experimental & Biomedical Science
2014 Volume.20 No. 2 p.70 ~ p.76
|
|
|
Anti-platelet Effects of Dimethyl Sulfoxide via Down-regulation of COX-1 and TXA₂ Synthase Activity in Rat Platelets
|
|
Ro Ju-Ye
Lee Hui-Jin
Ryu Jin-Hyeob
Park Hwa-Jin
Cho Hyun-Jeong
|
|
|
Abstract
|
|
|
|
In this study, we investigated the effect of DMSO, a highly dipolar organic liquid, in collagen (5 μg/ml)-stimulated platelet aggregation. DMSO inhibited platelet aggregation at 0.5% by inhibiting production of thromboxane A₂ (TXA₂) which was associated with blocking cyclooxygenase (COX)-1 activity and TXA₂ synthase. In addition, DMSO significantly increased the formation of cyclic adenosine monophosphate (cAMP) from adenosine triphosphate (ATP) and cyclic guanosine monophosphate (cGMP) from guanosine triphosphate (GTP). On the other hand, DMSO (0.1~0.5% concentration) did not affect the LDH release which indicates the cytotoxicity. Based on these results, DMSO has anti-platelet effect by regulation of several platelet signaling pathways, therefore we suggest that DMSO could be a novel strategy on many thrombotic disorders.
|
|
|
KEYWORD
|
|
|
DMSO, Platelet aggregation, cAMP, cGMP, COX-1 activity, TXA₂ synthase, TXA₂
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|